PN placement: three rows (branches ALP+UPS) — Class-3 PI3K complex-1 / BECN1-localization modulator; Chaperone-mediated autophagy|...|CMA enhancer|Enhancer of substrate uptake; and UPS|E3 ubiquitin and UBL ligases|Cul4A/Cul4B substrate receptor|WD40|other ; PN-node mapping: PI3K subtree context_only (too_broad, GO:0035032 / GO:0016236, no CC/process projection); CMA-enhancer type mapped→GO:1904716 (positive regulation of CMA); CRL4-receptor group mapped→GO:1990756 (UBL ligase-substrate adaptor). Projections: GO:1904716 more_specific_than_existing_goa, GO:1990756 already_in_goa_exact.
Consistency: Largely consistent on the UPS/autophagy axes; one divergence on CMA. CRL4 adaptor (GO:1990756) and autophagy/mitophagy roles match across deep research, review, PN, and mapping. The CMA-enhancer projection (GO:1904716) is NOT adopted by the review: it has no proposed_new_terms, and explicitly asks (suggested_question/experiment) whether AMBRA1 should be excluded from GO:1904716 until direct LAMP2A/HSPA8 substrate-uptake evidence exists. Review notes correctly state the AMBRA1 CMA-enhancer leaf (subtype) is no_mapping, but the dossier shows the parent type is mapped and projects GO:1904716 — so the projection still fires.
PN story / NEW pressure: Two PN MFs. GO:1990756 — already in GOA, abundant direct IDA support (PMID:23524951, 33854232/35/39, 30166453) → already captured (ACCEPT). GO:1904716 (verified real) — asserted via the CMA-enhancer bucket but supported only by the "CMA prevents neuronal proteome collapse" background paper, not AMBRA1-specific CMA substrate-uptake data. Verdict: GO:1990756 already captured; GO:1904716 over-reaches as a propagated annotation (candidate pending direct evidence).
Mapping strategy: PI3K subtree context_only is correctly conservative (no spurious GO:0035032 complex membership — avoids the TOMM20/RAB7A broader-than-review failure mode). The CMA-enhancer type→GO:1904716 mapping is the weak point: it propagates a positive-CMA process to AMBRA1 on thin evidence; review withholds it. CRL4 group→GO:1990756 is appropriately specific.
Recommended edits: [MAP] Downgrade the CMA-enhancer type→GO:1904716 to context_only (or mark AMBRA1 a gene-level exclusion) so positive-CMA is not auto-projected without LAMP2A/HSPA8 substrate-uptake evidence, consistent with the review's withholding.