VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and MVB12/UBAP-family partners. The best-supported cellular function is ESCRT-I-dependent endosomal sorting of ubiquitinated cargo into multivesicular bodies, with late-endosome/endosome-membrane localization and a secondary viral budding context. Current local evidence does not support transferring the VPS37A-specific phagophore-closure role to VPS37C as a core annotation.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | IBA GO_REF:0000033 | ACCEPT | Summary: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core VPS37C/ESCRT-I endosomal cargo-sorting role. Reason: VPS37C/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting into multivesicular bodies. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Required for the sorting of endocytic file:human/VPS37C/VPS37C-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15509564 required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies PMID:15509564 binds to another class E VPS factor, namely Hrs PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting |
| GO:0000813 ESCRT I complex | IBA GO_REF:0000033 | ACCEPT | Summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. Reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Component of the ESCRT-I complex file:human/VPS37C/VPS37C-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37C/VPS37C-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 PMID:15509564 Thus, this study identifies VPS37C as a functional component of mammalian ESCRT-I PMID:18005716 All ESCRT-I complexes contain three common subunits (TSG101, VPS28, and VPS37) PMID:22405001 complex with Vps23/TSG101, VPS28, and VPS37 |
| GO:0006612 protein targeting to membrane | IBA GO_REF:0000033 | MODIFY | Summary: protein targeting to membrane is too broad for VPS37C ESCRT-I function. Reason: The supported process is ESCRT-I-dependent endosomal cargo sorting into the MVB pathway, not generic protein targeting. Proposed replacements: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway multivesicular body assembly Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Required for the sorting of endocytic file:human/VPS37C/VPS37C-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15509564 required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies PMID:15509564 binds to another class E VPS factor, namely Hrs PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting |
| GO:0006623 protein targeting to vacuole | IBA GO_REF:0000033 | MODIFY | Summary: protein targeting to vacuole is too broad for VPS37C ESCRT-I function. Reason: The supported process is ESCRT-I-dependent endosomal cargo sorting into the MVB pathway, not generic protein targeting. Proposed replacements: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway multivesicular body assembly Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Required for the sorting of endocytic file:human/VPS37C/VPS37C-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15509564 required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies PMID:15509564 binds to another class E VPS factor, namely Hrs PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting |
| GO:0000813 ESCRT I complex | IEA GO_REF:0000117 | ACCEPT | Summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. Reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Component of the ESCRT-I complex file:human/VPS37C/VPS37C-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37C/VPS37C-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 PMID:15509564 Thus, this study identifies VPS37C as a functional component of mammalian ESCRT-I PMID:18005716 All ESCRT-I complexes contain three common subunits (TSG101, VPS28, and VPS37) PMID:22405001 complex with Vps23/TSG101, VPS28, and VPS37 |
| GO:0016236 macroautophagy | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Macroautophagy is over-annotated for VPS37C. Reason: The accessible ESCRT autophagy evidence is broad or VPS37A-specific; there is not enough direct evidence to make VPS37C a phagophore-closure/autophagosome assembly factor. Proposed replacements: ESCRT I complex Supporting Evidence: PMID:20588296 viral budding, cytokinesis and, probably, autophagy PMID:20588296 direct neck closure reaction in autophagy PMID:31519728 identify the ESCRT-I subunit VPS37A as a critical component PMID:31519728 required for autophagosome completion |
| GO:0031902 late endosome membrane | IEA GO_REF:0000044 | ACCEPT | Summary: late endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. Reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Late endosome membrane file:human/VPS37C/VPS37C-uniprot.txt Probably associates with membranes PMID:15509564 VPS37C is recruited to aberrant endosomes |
| GO:0036258 multivesicular body assembly | IEA GO_REF:0000117 | ACCEPT | Summary: multivesicular body assembly is supported as part of the core VPS37C/ESCRT-I endosomal cargo-sorting role. Reason: VPS37C/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting into multivesicular bodies. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Required for the sorting of endocytic file:human/VPS37C/VPS37C-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15509564 required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies PMID:15509564 binds to another class E VPS factor, namely Hrs PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting |
| GO:0039702 viral budding via host ESCRT complex | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Viral budding via host ESCRT complex is supported as a secondary context but is not the core VPS37C proteostasis function. Reason: VPS37C can support ESCRT-I-dependent viral budding, but the core cellular role is endosomal ESCRT-I cargo sorting. Supporting Evidence: PMID:15509564 VPS37C is recruited to the plasma membrane PMID:15509564 direct fusion of VPS37C to HIV-1 Gag PMID:15509564 inhibited by VPS37C depletion PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:20588296 viral budding, cytokinesis and, probably, autophagy |
| GO:0048306 calcium-dependent protein binding | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Calcium-dependent protein binding is supported but secondary. Reason: VPS37C participates in an ALG-2/ALIX/ESCRT-I calcium-dependent ternary-complex context, but this is not the core VPS37C proteostasis function. Supporting Evidence: PMID:23924735 VPS37B and VPS37C appeared to interact with ALG-2 PMID:23924735 adaptor protein that bridges ALIX and ESCRT-I |
| GO:0005515 protein binding | IPI PMID:17853893 Human ESCRT and ALIX proteins interact with proteins of the ... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37C function. Reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. Proposed replacements: ESCRT I complex Supporting Evidence: PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 file:human/VPS37C/VPS37C-notes.md Generic `protein binding` rows are over-annotated |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37C function. Reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. Proposed replacements: ESCRT I complex Supporting Evidence: PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 file:human/VPS37C/VPS37C-notes.md Generic `protein binding` rows are over-annotated |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37C function. Reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. Proposed replacements: ESCRT I complex Supporting Evidence: PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 file:human/VPS37C/VPS37C-notes.md Generic `protein binding` rows are over-annotated |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37C function. Reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. Proposed replacements: ESCRT I complex Supporting Evidence: PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 file:human/VPS37C/VPS37C-notes.md Generic `protein binding` rows are over-annotated |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37C function. Reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. Proposed replacements: ESCRT I complex Supporting Evidence: PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 file:human/VPS37C/VPS37C-notes.md Generic `protein binding` rows are over-annotated |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: Nucleoplasm localization is not part of the core VPS37C ESCRT-I function. Reason: The core supported localization is late endosome/endosome membrane; the nucleoplasm row comes from high-throughput localization mapping and should not drive the functional model. Supporting Evidence: file:human/VPS37C/VPS37C-notes.md Extracellular exosome and nucleoplasm rows come from high-throughput localization/proteomics and should remain non-core |
| GO:0000813 ESCRT I complex | IPI PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t... | ACCEPT | Summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. Reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Component of the ESCRT-I complex file:human/VPS37C/VPS37C-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37C/VPS37C-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 PMID:15509564 Thus, this study identifies VPS37C as a functional component of mammalian ESCRT-I PMID:18005716 All ESCRT-I complexes contain three common subunits (TSG101, VPS28, and VPS37) PMID:22405001 complex with Vps23/TSG101, VPS28, and VPS37 |
| GO:0000813 ESCRT I complex | IPI PMID:21757351 UBAP1 is a component of an endosome-specific ESCRT-I complex... | ACCEPT | Summary: ESCRT-I complex membership is correct for VPS37C, but PMID:21757351 is not the strongest VPS37C-specific support. Reason: The term is correct from VPS37C-specific and shared ESCRT-I evidence; however, PMID:21757351 specifically defines a UBAP1/VPS37A endosome-specific complex and says it does not contain VPS37C. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Component of the ESCRT-I complex file:human/VPS37C/VPS37C-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37C/VPS37C-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 PMID:15509564 Thus, this study identifies VPS37C as a functional component of mammalian ESCRT-I PMID:21757351 contains VPS37A but not VPS37C |
| GO:0010008 endosome membrane | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: endosome membrane is correct for VPS37C, but PMID:32424346 is indirect for this isoform. Reason: Endosome-membrane localization is supported by VPS37C-specific evidence; PMID:32424346 supports general ESCRT-I mechanism using a VPS37B-containing headpiece. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Late endosome membrane file:human/VPS37C/VPS37C-uniprot.txt Probably associates with membranes PMID:15509564 VPS37C is recruited to aberrant endosomes PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A |
| GO:0036258 multivesicular body assembly | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: multivesicular body assembly is supported as part of the core VPS37C/ESCRT-I endosomal cargo-sorting role. Reason: VPS37C/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting into multivesicular bodies. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Required for the sorting of endocytic file:human/VPS37C/VPS37C-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15509564 required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies PMID:15509564 binds to another class E VPS factor, namely Hrs PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting |
| GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core VPS37C/ESCRT-I endosomal cargo-sorting role. Reason: VPS37C/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting into multivesicular bodies. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Required for the sorting of endocytic file:human/VPS37C/VPS37C-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15509564 required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies PMID:15509564 binds to another class E VPS factor, namely Hrs PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting |
| GO:0090148 membrane fission | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | MARK AS OVER ANNOTATED | Summary: Membrane fission is a broad ESCRT-I mechanism annotation and is over-transferred for VPS37C as written. Reason: The cited structural/autophagy work directly tested a VPS37B-containing headpiece and VPS28 interface mutants, not VPS37C; the safer VPS37C annotations are ESCRT-I complex and MVB sorting. Proposed replacements: ESCRT I complex multivesicular body assembly Supporting Evidence: PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A PMID:32424346 ESCRT-I is not merely a bridging adaptor PMID:15509564 functional component of mammalian ESCRT-I |
| GO:0016236 macroautophagy | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | MARK AS OVER ANNOTATED | Summary: Macroautophagy is over-annotated for VPS37C. Reason: The accessible ESCRT autophagy evidence is broad or VPS37A-specific; there is not enough direct evidence to make VPS37C a phagophore-closure/autophagosome assembly factor. Proposed replacements: ESCRT I complex Supporting Evidence: PMID:20588296 viral budding, cytokinesis and, probably, autophagy PMID:20588296 direct neck closure reaction in autophagy PMID:31519728 identify the ESCRT-I subunit VPS37A as a critical component PMID:31519728 required for autophagosome completion |
| GO:0000813 ESCRT I complex | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. Reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Component of the ESCRT-I complex file:human/VPS37C/VPS37C-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37C/VPS37C-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 PMID:15509564 Thus, this study identifies VPS37C as a functional component of mammalian ESCRT-I PMID:18005716 All ESCRT-I complexes contain three common subunits (TSG101, VPS28, and VPS37) PMID:22405001 complex with Vps23/TSG101, VPS28, and VPS37 |
| GO:0036258 multivesicular body assembly | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: multivesicular body assembly is supported as part of the core VPS37C/ESCRT-I endosomal cargo-sorting role. Reason: VPS37C/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting into multivesicular bodies. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Required for the sorting of endocytic file:human/VPS37C/VPS37C-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15509564 required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies PMID:15509564 binds to another class E VPS factor, namely Hrs PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting |
| GO:0039702 viral budding via host ESCRT complex | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | KEEP AS NON CORE | Summary: Viral budding via host ESCRT complex is supported as a secondary context but is not the core VPS37C proteostasis function. Reason: VPS37C can support ESCRT-I-dependent viral budding, but the core cellular role is endosomal ESCRT-I cargo sorting. Supporting Evidence: PMID:15509564 VPS37C is recruited to the plasma membrane PMID:15509564 direct fusion of VPS37C to HIV-1 Gag PMID:15509564 inhibited by VPS37C depletion PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:20588296 viral budding, cytokinesis and, probably, autophagy |
| GO:0000813 ESCRT I complex | IDA PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t... | ACCEPT | Summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. Reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Component of the ESCRT-I complex file:human/VPS37C/VPS37C-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37C/VPS37C-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 PMID:15509564 Thus, this study identifies VPS37C as a functional component of mammalian ESCRT-I PMID:18005716 All ESCRT-I complexes contain three common subunits (TSG101, VPS28, and VPS37) PMID:22405001 complex with Vps23/TSG101, VPS28, and VPS37 |
| GO:0005515 protein binding | IPI PMID:23924735 VPS37 isoforms differentially modulate the ternary complex f... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37C function. Reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. Proposed replacements: calcium-dependent protein binding Supporting Evidence: PMID:23924735 VPS37B and VPS37C appeared to interact with ALG-2 PMID:23924735 adaptor protein that bridges ALIX and ESCRT-I |
| GO:0048306 calcium-dependent protein binding | IPI PMID:23924735 VPS37 isoforms differentially modulate the ternary complex f... | KEEP AS NON CORE | Summary: Calcium-dependent protein binding is supported but secondary. Reason: VPS37C participates in an ALG-2/ALIX/ESCRT-I calcium-dependent ternary-complex context, but this is not the core VPS37C proteostasis function. Supporting Evidence: PMID:23924735 VPS37B and VPS37C appeared to interact with ALG-2 PMID:23924735 adaptor protein that bridges ALIX and ESCRT-I |
| GO:0070062 extracellular exosome | HDA PMID:23533145 In-depth proteomic analyses of exosomes isolated from expres... | KEEP AS NON CORE | Summary: Extracellular exosome is a high-throughput proteomics/localization context and not a core VPS37C annotation. Reason: Exosome detection is not the central VPS37C ESCRT-I endosomal sorting function. Supporting Evidence: PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exosomes PMID:23533145 In-depth proteomic analyses of exosomes |
| GO:0070062 extracellular exosome | HDA PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exos... | KEEP AS NON CORE | Summary: Extracellular exosome is a high-throughput proteomics/localization context and not a core VPS37C annotation. Reason: Exosome detection is not the central VPS37C ESCRT-I endosomal sorting function. Supporting Evidence: PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exosomes PMID:23533145 In-depth proteomic analyses of exosomes |
| GO:0000813 ESCRT I complex | IDA PMID:22405001 The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a ... | ACCEPT | Summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. Reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Component of the ESCRT-I complex file:human/VPS37C/VPS37C-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37C/VPS37C-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15509564 VPS37C can form a ternary complex with Tsg101 and VPS28 PMID:15509564 Thus, this study identifies VPS37C as a functional component of mammalian ESCRT-I PMID:18005716 All ESCRT-I complexes contain three common subunits (TSG101, VPS28, and VPS37) PMID:22405001 complex with Vps23/TSG101, VPS28, and VPS37 |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-184269 | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. Reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Late endosome membrane file:human/VPS37C/VPS37C-uniprot.txt Probably associates with membranes PMID:15509564 VPS37C is recruited to aberrant endosomes |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-3149434 | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. Reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Late endosome membrane file:human/VPS37C/VPS37C-uniprot.txt Probably associates with membranes PMID:15509564 VPS37C is recruited to aberrant endosomes |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-3159232 | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. Reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Late endosome membrane file:human/VPS37C/VPS37C-uniprot.txt Probably associates with membranes PMID:15509564 VPS37C is recruited to aberrant endosomes |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-917696 | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. Reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Late endosome membrane file:human/VPS37C/VPS37C-uniprot.txt Probably associates with membranes PMID:15509564 VPS37C is recruited to aberrant endosomes |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-917730 | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. Reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. Supporting Evidence: file:human/VPS37C/VPS37C-uniprot.txt Late endosome membrane file:human/VPS37C/VPS37C-uniprot.txt Probably associates with membranes PMID:15509564 VPS37C is recruited to aberrant endosomes |
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Download this section (compressed HTML)Q: Should VPS37C macroautophagy annotations be retired or kept non-core given that the direct phagophore-closure evidence is VPS37A-specific?
Suggested experts: GO autophagy editors, GO ESCRT curators
Q: Should generic VPS37C protein-binding annotations be replaced by ESCRT-I complex membership, calcium-dependent ALG-2 interaction context, and specific endosomal sorting terms?
Suggested experts: GO molecular function editors, UniProt curators
Experiment: Compare VPS37A, VPS37B, and VPS37C depletion/rescue in HT-LC3 autophagosome closure assays and parallel EGFR or tetherin MVB-sorting assays, using endogenous expression or matched rescue levels.
Hypothesis: VPS37A, but not necessarily VPS37C, is the VPS37 paralog specialized for phagophore closure.
Type: VPS37 paralog phagophore closure comparison
Experiment: Use VPS37C-specific knockout-rescue with quantitative cargo degradation and HIV-1 Gag recruitment/release readouts, controlling for VPS37A and VPS37B compensation.
Hypothesis: VPS37C contributes to a subset of ESCRT-I cargo-sorting or viral-budding contexts distinct from the UBAP1/VPS37A endosome-specific complex.
Type: VPS37C-specific MVB sorting assay
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