RNF185

RNF185

• UniProt: Q96GF1 · batch: proteostasis-batch-2026-06-11 · review status: COMPLETE (very thorough; ER RING E3, ERAD/CFTR core + BNIP1 mitophagy, cGAS/K27, Membralin/Tapasin)
• PN placement: 4 rows — ER proteostasis|...|ERAD|...|ERAD-associated RING E3 ligase; ALP|Autophagy substrate selection|Autophagy receptor regulation|Mitophagy; UPS|...|RING|with transmembrane domain|ER, mitochondria; UPS|...|idiosyncratic RING complex|membralin complex|catalytic / RING, transmembrane. PN-node mapping: ERAD-RING subtype→GO:0061630 (in_goa); ERAD type/group→GO:0036503 (in_goa); Mitophagy type→GO:0000423 mitophagy (new_to_goa); RING group→GO:0061630 (in_goa); membralin-complex group→GO:0000151 ubiquitin ligase complex (new_to_goa). Projected: GO:0036503×2, GO:0061630×2 (in GOA), GO:0000423 (new), GO:0000151 (new).
• Consistency: Excellent. Deep research, review and PN concur: ER RING E3 (Cys-39), CFTR/ΔF508 ERAD redundant with RNF5; non-core BNIP1 K63 mitophagy (PMID:21931693), cGAS K27/antiviral (PMID:28273161), and the RNF185/Membralin/TMUB1-2 ER-membrane ligase complex. PN row 4 reference "32738194" verified via PubMed = van de Weijer et al. 2020 Mol Cell "Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex" (DOI) — the same complex the review cites via PMID:39353943 (Tapasin/Membralin). No contradictions.
• PN story / NEW pressure: GO:0061630 + GO:0036503 already in GOA/review. GO:0000423 mitophagy (verified real) is new_to_goa — RNF185's BNIP1 role supports selective mitochondrial autophagy, a DEFENSIBLE ADD (review captures it only as BNIP1-substrate ligase activity + K63, no mitophagy BP term). GO:0000151 ubiquitin ligase complex (verified real, NOT in GOA) is well-grounded by the Membralin complex (PMID:32738194/39353943) — DEFENSIBLE ADD; review has GO:0044877 (complex binding) but no complex CC term. Conclude: catalytic/ERAD captured; GO:0000423 and GO:0000151 both defensible NEW.
• Mapping strategy: Correct and conservative. Membralin-complex group→GO:0000151 (complex membership, not catalytic activity to every subunit) is sound; consider the more specific child GO:0000835 ER ubiquitin ligase complex (verified in OLS) given the ER-membrane context. Mitophagy via receptor-regulation framing → GO:0000423 appropriate. No node-status change warranted.
• Evidence alignment: Strong. PN mitophagy row PMID:21931693 = review BNIP1 annotation; PN membralin PMID:32738194 = review's Membralin/Tapasin literature (PMID:39353943, also van de Weijer). PN RING row "19489725 / rev" not in review (family review; low impact).
• Verdict: Fully consistent; ligase MF + ERAD already captured, GO:0000423 mitophagy and GO:0000151 ubiquitin ligase complex both defensible NEW (non-core). No YAML change strictly required.
• Recommended edits: [YAML] (optional) add GO:0000151 ubiquitin ligase complex (or child GO:0000835 ER ubiquitin ligase complex; part_of, PMID:32738194) and GO:0000423 mitophagy (involved_in, PMID:21931693) as non-core annotations to mirror the PN projections. [MAP] consider GO:0000835 ER ubiquitin ligase complex as a more precise target for the membralin-complex node.