UniProt: Q99942 · batch: proteostasis-batch-2026-06-11 · review status: COMPLETE (very thorough; tail-anchored ER RING E3, ERAD core + STING/ATG4B/paxillin moonlighting)
PN placement: 3 rows — ER proteostasis|...|ER associated degradation|Cytosolic handling of ERAD substrates|ERAD-associated RING E3 ligase; ALP|Autophagophore initiation and elongation|...|Modulation of ATG4 activity; UPS|E3 ubiquitin and UBL ligases|RING|with transmembrane domain|ER, mitochondria, cell membrane. PN-node mapping: ERAD-RING-E3 subtype→GO:0061630 (in_goa); ERAD type/group→GO:0036503 (in_goa); ATG4-modulation subtype→GO:2000785 regulation of autophagosome assembly (new_to_goa); RING group→GO:0061630 (in_goa). Projected: GO:0036503×2, GO:0061630×2 (all in GOA), GO:2000785 (new).
Consistency: Excellent. Deep research, review and PN concur on the core: ER-membrane RING E3 (Cys-42), ERAD of CFTR/ΔF508 (K48), partly redundant with RNF185; plus the non-core ATG4B/autophagy, STING1/MITA antiviral (K48, mitochondria), JAMP (K63), and paxillin/motility roles. The PN ATG4 row's note ("controls stability of ATG4B") and its reference (PMID:23093945, ATG4B/RNF5 PLOS paper) match the review's GO:0061630 EXP annotation on PMID:23093945. No contradictions.
PN story / NEW pressure:GO:0061630 + GO:0036503 already in GOA and ACCEPTed (multiple EXP/IMP/IGI). GO:2000785 regulation of autophagosome assembly (verified real, OLS) is new_to_goa — RNF5 genuinely limits basal autophagy via ATG4B stability, so this is a DEFENSIBLE ADD, though it is a regulatory framing of a role the review treats as non-core moonlighting (the review captures ATG4B only as substrate-directed ligase activity, no autophagy-process term). Conclude: ligase MF + ERAD already captured; GO:2000785 defensible NEW (non-core).
Mapping strategy: Correct. Catalytic RING E3 → GO:0061630 at subtype/group; ERAD pathway at type/group; subtype-with-TM no_mapping avoids double-counting. ATG4-modulation leaf → GO:2000785 is appropriately conservative (regulation, not core autophagy). No node-status change warranted.
Evidence alignment: Strong overlap. PN ATG4 row PMID:23093945 = review's autophagy annotation. PN RING row cites "19489725 / rev" (Kaneko/RING-family review) — NOT in the review's reference list (review uses gene-specific EXP papers instead); low impact since ligase MF is independently well-supported.
Verdict: Fully consistent; PN ligase/ERAD already captured, GO:2000785 a defensible (non-core) NEW autophagy-regulation term. No YAML change required.
Recommended edits: none required. [YAML] (optional) consider adding GO:2000785 regulation of autophagosome assembly (IMP/IDA, PMID:23093945) as a non-core BP to mirror the PN projection. [REF] PN-cited PMID:19489725 (RING row) absent from review — verify it is a family review, not gene-specific.