ADRM1 encodes the human proteasomal ubiquitin receptor Rpn13, a 19S regulatory-particle subunit/cofactor of the 26S proteasome. Its N-terminal Pru domain binds ubiquitin signals and the Rpn2/PSMD1 proteasome scaffold, while its C-terminal DEUBAD region binds the UCHL5/UCH37 deubiquitinase. Through these interactions ADRM1 helps recruit ubiquitinated substrates and coordinate deubiquitinase activity during proteasome-mediated protein degradation in cytosolic and nuclear proteostasis.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0008541 proteasome regulatory particle, lid subcomplex | IBA GO_REF:0000033 | MODIFY | Summary: The evidence supports ADRM1/Rpn13 as a 19S regulatory-particle ubiquitin receptor, but the lid-subcomplex placement is less accurate for ADRM1 than the base-subcomplex placement used by the PN projection. Reason: Modify to proteasome regulatory particle, base subcomplex. Primary and structural literature describe Rpn13/ADRM1 as a 19S regulatory-particle subunit and as one of the base ubiquitin receptors; this supports the PN base-subcomplex projection and argues against retaining the lid-subcomplex term for ADRM1. Proposed replacements: proteasome regulatory particle, base subcomplex proteasome regulatory particle Supporting Evidence: PMID:17139257 we discovered a novel 46-kDa (407 residues) subunit of its 19S regulatory complex PMID:29636472 Recognition of a ubiquitylated substrate is mediated principally by ubiquitin receptors Rpn10 and Rpn13, the base subunits within the holoenzyme PMID:33729481 Three of these integral subunits (Rpn1, Rpn10 and Rpn13) bind to the conjugated ubiquitin tag on the substrate file:human/ADRM1/ADRM1-deep-research-manual.md The Proteostasis PN projection is conservative for ADRM1; proteasome complex and regulatory-particle terms are safe, and literature supports modifying the lid-subcomplex annotation to the base subcomplex. |
| GO:0061133 endopeptidase activator activity | IBA GO_REF:0000033 | MODIFY | Summary: ADRM1 activates the proteasome-associated deubiquitinase UCHL5/UCH37, so the current endopeptidase activator term is too generic and mechanistically imprecise. Reason: Modify to deubiquitinase activator activity. The cited evidence is about recruiting/activating UCH37/UCHL5 deubiquitinating activity at 26S proteasomes, not a broad endopeptidase activator role. Proposed replacements: deubiquitinase activator activity Supporting Evidence: PMID:16990800 hRpn13 recruits UCH37, a deubiquitinating enzyme known to associate with 26 proteasomes PMID:16990800 loss of UCH37 proteins and decrease in deubiquitinating activity of 26S proteasomes PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0070628 proteasome binding | IBA GO_REF:0000033 | ACCEPT | Summary: ADRM1 binds/incorporates into the 26S proteasome regulatory particle through its Rpn13/Rpn2-associated receptor role. Reason: Accept as a direct molecular function. ADRM1 is a proteasome-associated ubiquitin receptor and interacts with the proteasome scaffolding protein Rpn2/PSMD1; this is also consistent with the PN regulatory-particle projection. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:17139257 we discovered a novel 46-kDa (407 residues) subunit of its 19S regulatory complex PMID:20471946 hRpn13 binding to the proteasome scaffolding protein hRpn2/S1 abrogates its interdomain interactions, thus activating hRpn13 for ubiquitin binding |
| GO:0005634 nucleus | IEA GO_REF:0000120 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005515 protein binding | IPI PMID:16713569 A protein-protein interaction network for human inherited at... | MARK AS OVER ANNOTATED | Summary: This generic protein-binding annotation comes from interaction evidence, but generic protein binding is uninformative for ADRM1. Reason: Mark as over-annotated. ADRM1 has specific, better-supported binding functions as a proteasomal ubiquitin receptor, a proteasome/Rpn2-associated protein, and a UCHL5-binding deubiquitinase regulator. Screen-derived or duplicate protein-binding rows should not be retained as core molecular functions. Supporting Evidence: PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:16990800 A novel proteasome interacting protein recruits the deubiqui... | MODIFY | Summary: This protein-binding evidence is real but should be represented by more specific proteasome binding and UCHL5/UCH37 binding terms. Reason: Modify away from generic protein binding. The original evidence supports ADRM1 association with proteasome subunits and the UCHL5/UCH37 deubiquitinase, which are better captured by proteasome binding and ubiquitin-specific protease binding/deubiquitinase-regulator annotations. Proposed replacements: proteasome binding ubiquitin-specific protease binding Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:16990800 hRpn13 recruits UCH37, a deubiquitinating enzyme known to associate with 26 proteasomes PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:17139257 hRpn13/ADRM1/GP110 is a novel proteasome subunit that binds ... | MODIFY | Summary: This protein-binding evidence is real but should be represented by more specific proteasome binding and UCHL5/UCH37 binding terms. Reason: Modify away from generic protein binding. The original evidence supports ADRM1 association with proteasome subunits and the UCHL5/UCH37 deubiquitinase, which are better captured by proteasome binding and ubiquitin-specific protease binding/deubiquitinase-regulator annotations. Proposed replacements: proteasome binding ubiquitin-specific protease binding Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:16990800 hRpn13 recruits UCH37, a deubiquitinating enzyme known to associate with 26 proteasomes PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:18497817 Proteasome subunit Rpn13 is a novel ubiquitin receptor. | MODIFY | Summary: This protein-binding row captures direct ubiquitin/UBL-substrate receptor evidence and should be replaced by ubiquitin binding. Reason: Modify to ubiquitin binding. Husnjak et al. identify Rpn13/ADRM1 as a proteasomal ubiquitin receptor and map ubiquitin binding to the Pru domain; generic protein binding hides the informative molecular function. Proposed replacements: ubiquitin binding Supporting Evidence: PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:20471946 Rpn13 functions as a ubiquitin receptor for the proteasome |
| GO:0005515 protein binding | IPI PMID:18922472 Distinct modes of regulation of the Uch37 deubiquitinating e... | MARK AS OVER ANNOTATED | Summary: This generic protein-binding annotation comes from interaction evidence, but generic protein binding is uninformative for ADRM1. Reason: Mark as over-annotated. ADRM1 has specific, better-supported binding functions as a proteasomal ubiquitin receptor, a proteasome/Rpn2-associated protein, and a UCHL5-binding deubiquitinase regulator. Screen-derived or duplicate protein-binding rows should not be retained as core molecular functions. Supporting Evidence: PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:19490896 Assembly pathway of the Mammalian proteasome base subcomplex... | MARK AS OVER ANNOTATED | Summary: This generic protein-binding annotation comes from interaction evidence, but generic protein binding is uninformative for ADRM1. Reason: Mark as over-annotated. ADRM1 has specific, better-supported binding functions as a proteasomal ubiquitin receptor, a proteasome/Rpn2-associated protein, and a UCHL5-binding deubiquitinase regulator. Screen-derived or duplicate protein-binding rows should not be retained as core molecular functions. Supporting Evidence: PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:19615732 Defining the human deubiquitinating enzyme interaction lands... | MARK AS OVER ANNOTATED | Summary: This generic protein-binding annotation comes from interaction evidence, but generic protein binding is uninformative for ADRM1. Reason: Mark as over-annotated. ADRM1 has specific, better-supported binding functions as a proteasomal ubiquitin receptor, a proteasome/Rpn2-associated protein, and a UCHL5-binding deubiquitinase regulator. Screen-derived or duplicate protein-binding rows should not be retained as core molecular functions. Supporting Evidence: PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:20059542 Cross-species divergence of the major recognition pathways o... | MODIFY | Summary: This generic protein-binding annotation reflects ADRM1 receptor/shuttle-factor or proteasome-subunit interactions, but those should be represented with more specific terms. Reason: Modify to more informative binding terms where the evidence supports them. ADRM1 directly recognizes ubiquitin and associates with the proteasome/Rpn2 context; generic protein binding is too broad for curation. Proposed replacements: ubiquitin binding proteasome binding Supporting Evidence: PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:20471946 hRpn13 binding to the proteasome scaffolding protein hRpn2/S1 abrogates its interdomain interactions, thus activating hRpn13 for ubiquitin binding PMID:24811749 Rpn13 ubiquitination strongly decreases the proteasome's ability to bind and degrade ubiquitin-conjugated proteins |
| GO:0005515 protein binding | IPI PMID:21516116 Next-generation sequencing to generate interactome datasets. | MARK AS OVER ANNOTATED | Summary: This generic protein-binding annotation comes from interaction evidence, but generic protein binding is uninformative for ADRM1. Reason: Mark as over-annotated. ADRM1 has specific, better-supported binding functions as a proteasomal ubiquitin receptor, a proteasome/Rpn2-associated protein, and a UCHL5-binding deubiquitinase regulator. Screen-derived or duplicate protein-binding rows should not be retained as core molecular functions. Supporting Evidence: PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:24811749 Autoubiquitination of the 26S proteasome on Rpn13 regulates ... | MODIFY | Summary: This generic protein-binding annotation reflects ADRM1 receptor/shuttle-factor or proteasome-subunit interactions, but those should be represented with more specific terms. Reason: Modify to more informative binding terms where the evidence supports them. ADRM1 directly recognizes ubiquitin and associates with the proteasome/Rpn2 context; generic protein binding is too broad for curation. Proposed replacements: ubiquitin binding proteasome binding Supporting Evidence: PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:20471946 hRpn13 binding to the proteasome scaffolding protein hRpn2/S1 abrogates its interdomain interactions, thus activating hRpn13 for ubiquitin binding PMID:24811749 Rpn13 ubiquitination strongly decreases the proteasome's ability to bind and degrade ubiquitin-conjugated proteins |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: This generic protein-binding annotation comes from interaction evidence, but generic protein binding is uninformative for ADRM1. Reason: Mark as over-annotated. ADRM1 has specific, better-supported binding functions as a proteasomal ubiquitin receptor, a proteasome/Rpn2-associated protein, and a UCHL5-binding deubiquitinase regulator. Screen-derived or duplicate protein-binding rows should not be retained as core molecular functions. Supporting Evidence: PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:27107012 Pooled-matrix protein interaction screens using Barcode Fusi... | MARK AS OVER ANNOTATED | Summary: This generic protein-binding annotation comes from interaction evidence, but generic protein binding is uninformative for ADRM1. Reason: Mark as over-annotated. ADRM1 has specific, better-supported binding functions as a proteasomal ubiquitin receptor, a proteasome/Rpn2-associated protein, and a UCHL5-binding deubiquitinase regulator. Screen-derived or duplicate protein-binding rows should not be retained as core molecular functions. Supporting Evidence: PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MARK AS OVER ANNOTATED | Summary: This generic protein-binding annotation comes from interaction evidence, but generic protein binding is uninformative for ADRM1. Reason: Mark as over-annotated. ADRM1 has specific, better-supported binding functions as a proteasomal ubiquitin receptor, a proteasome/Rpn2-associated protein, and a UCHL5-binding deubiquitinase regulator. Screen-derived or duplicate protein-binding rows should not be retained as core molecular functions. Supporting Evidence: PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:31064842 Phosphorylation of Tyr-950 in the proteasome scaffolding pro... | MODIFY | Summary: This generic protein-binding annotation reflects ADRM1 receptor/shuttle-factor or proteasome-subunit interactions, but those should be represented with more specific terms. Reason: Modify to more informative binding terms where the evidence supports them. ADRM1 directly recognizes ubiquitin and associates with the proteasome/Rpn2 context; generic protein binding is too broad for curation. Proposed replacements: ubiquitin binding proteasome binding Supporting Evidence: PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:20471946 hRpn13 binding to the proteasome scaffolding protein hRpn2/S1 abrogates its interdomain interactions, thus activating hRpn13 for ubiquitin binding PMID:24811749 Rpn13 ubiquitination strongly decreases the proteasome's ability to bind and degrade ubiquitin-conjugated proteins |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: This generic protein-binding annotation comes from interaction evidence, but generic protein binding is uninformative for ADRM1. Reason: Mark as over-annotated. ADRM1 has specific, better-supported binding functions as a proteasomal ubiquitin receptor, a proteasome/Rpn2-associated protein, and a UCHL5-binding deubiquitinase regulator. Screen-derived or duplicate protein-binding rows should not be retained as core molecular functions. Supporting Evidence: PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MARK AS OVER ANNOTATED | Summary: This generic protein-binding annotation comes from interaction evidence, but generic protein binding is uninformative for ADRM1. Reason: Mark as over-annotated. ADRM1 has specific, better-supported binding functions as a proteasomal ubiquitin receptor, a proteasome/Rpn2-associated protein, and a UCHL5-binding deubiquitinase regulator. Screen-derived or duplicate protein-binding rows should not be retained as core molecular functions. Supporting Evidence: PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0005515 protein binding | IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func... | MARK AS OVER ANNOTATED | Summary: This generic protein-binding annotation comes from interaction evidence, but generic protein binding is uninformative for ADRM1. Reason: Mark as over-annotated. ADRM1 has specific, better-supported binding functions as a proteasomal ubiquitin receptor, a proteasome/Rpn2-associated protein, and a UCHL5-binding deubiquitinase regulator. Screen-derived or duplicate protein-binding rows should not be retained as core molecular functions. Supporting Evidence: PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0000502 proteasome complex | NAS PMID:29636472 Structural mechanism for nucleotide-driven remodeling of the... | ACCEPT | Summary: ADRM1 is a component of the 26S proteasome/regulatory-particle complex and is repeatedly recovered in proteasome structural and biochemical studies. Reason: Accept. Although the more precise PN-supported complex placement is the regulatory-particle base subcomplex, proteasome complex is a true broader cellular-component annotation for ADRM1. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:17139257 we discovered a novel 46-kDa (407 residues) subunit of its 19S regulatory complex PMID:29636472 Recognition of a ubiquitylated substrate is mediated principally by ubiquitin receptors Rpn10 and Rpn13, the base subunits within the holoenzyme PMID:33729481 Three of these integral subunits (Rpn1, Rpn10 and Rpn13) bind to the conjugated ubiquitin tag on the substrate |
| GO:0000502 proteasome complex | NAS PMID:33729481 Proteasome in action: substrate degradation by the 26S prote... | ACCEPT | Summary: ADRM1 is a component of the 26S proteasome/regulatory-particle complex and is repeatedly recovered in proteasome structural and biochemical studies. Reason: Accept. Although the more precise PN-supported complex placement is the regulatory-particle base subcomplex, proteasome complex is a true broader cellular-component annotation for ADRM1. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:17139257 we discovered a novel 46-kDa (407 residues) subunit of its 19S regulatory complex PMID:29636472 Recognition of a ubiquitylated substrate is mediated principally by ubiquitin receptors Rpn10 and Rpn13, the base subunits within the holoenzyme PMID:33729481 Three of these integral subunits (Rpn1, Rpn10 and Rpn13) bind to the conjugated ubiquitin tag on the substrate |
| GO:0000502 proteasome complex | NAS PMID:37228199 An abundance of free regulatory (19S) proteasome particles r... | ACCEPT | Summary: ADRM1 is a component of the 26S proteasome/regulatory-particle complex and is repeatedly recovered in proteasome structural and biochemical studies. Reason: Accept. Although the more precise PN-supported complex placement is the regulatory-particle base subcomplex, proteasome complex is a true broader cellular-component annotation for ADRM1. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:17139257 we discovered a novel 46-kDa (407 residues) subunit of its 19S regulatory complex PMID:29636472 Recognition of a ubiquitylated substrate is mediated principally by ubiquitin receptors Rpn10 and Rpn13, the base subunits within the holoenzyme PMID:33729481 Three of these integral subunits (Rpn1, Rpn10 and Rpn13) bind to the conjugated ubiquitin tag on the substrate |
| GO:0005829 cytosol | NAS PMID:12032076 Properties of the hybrid form of the 26S proteasome containi... | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0006979 response to oxidative stress | NAS PMID:35858375 Structural insights into the human PA28-20S proteasome enabl... | MARK AS OVER ANNOTATED | Summary: The cited PA28-20S structural work does not provide ADRM1-specific oxidative-stress function. Reason: Mark as over-annotated. Proteasome capacity and ADRM1/Rpn13 autoubiquitination can respond to proteotoxic stress, but the GOA-cited PA28-20S paper does not make ADRM1 a direct response-to-oxidative-stress gene product. Supporting Evidence: PMID:24811749 Rpn13 becomes extensively and selectively poly-ubiquitinated by the proteasome-associated ubiquitin ligase, Ube3c/Hul5 PMID:24811749 Rpn13 ubiquitination strongly decreases the proteasome's ability to bind and degrade ubiquitin-conjugated proteins |
| GO:0008021 synaptic vesicle | NAS PMID:37228199 An abundance of free regulatory (19S) proteasome particles r... | MARK AS OVER ANNOTATED | Summary: The cited work supports free 19S regulatory particles near synapses, but not ADRM1 as a synaptic-vesicle component. Reason: Mark as over-annotated. A 19S regulatory-particle pool at synapses is relevant proteasome biology, but synaptic vesicle is too specific for ADRM1 without direct ADRM1-resolved localization evidence. Supporting Evidence: PMID:37228199 unexpected abundance of free 19S particles near synapses |
| GO:0010498 proteasomal protein catabolic process | NAS PMID:33729481 Proteasome in action: substrate degradation by the 26S prote... | ACCEPT | Summary: ADRM1 contributes to proteasomal protein catabolism by recognizing ubiquitinated substrates as Rpn13 and supporting proteasome-associated UCHL5 activity. Reason: Accept as a direct proteostasis process at an appropriate broad level. ADRM1 is not the protease catalytic subunit, but its ubiquitin-receptor and UCHL5-regulatory roles are part of proteasomal substrate processing. Supporting Evidence: PMID:17139257 Knockdown of hRpn13 in 293T cells increases the cellular levels of ubiquitin conjugates and decreases the degradation of short-lived proteins PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:20471946 When wild-type hRpn13 was added to these proteasomes, the degradation of ubiquitinated cyclin B was strongly stimulated PMID:33729481 Proteasomes are also vital for maintaining intracellular protein quality control by removing misfolded or aggregate-prone damaged proteins |
| GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process | NAS PMID:19489727 Recognition and processing of ubiquitin-protein conjugates b... | ACCEPT | Summary: ADRM1/Rpn13 functions in ubiquitin-dependent proteasomal degradation by binding ubiquitin signals and supporting 26S proteasome substrate processing. Reason: Accept as a core biological process. Multiple primary studies and reviews support ADRM1 as a 26S proteasome ubiquitin receptor required for efficient degradation of ubiquitin conjugates. Supporting Evidence: PMID:17139257 Knockdown of hRpn13 in 293T cells increases the cellular levels of ubiquitin conjugates and decreases the degradation of short-lived proteins PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:20471946 When wild-type hRpn13 was added to these proteasomes, the degradation of ubiquitinated cyclin B was strongly stimulated PMID:33729481 Three of these integral subunits (Rpn1, Rpn10 and Rpn13) bind to the conjugated ubiquitin tag on the substrate |
| GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process | NAS PMID:33729481 Proteasome in action: substrate degradation by the 26S prote... | ACCEPT | Summary: ADRM1/Rpn13 functions in ubiquitin-dependent proteasomal degradation by binding ubiquitin signals and supporting 26S proteasome substrate processing. Reason: Accept as a core biological process. Multiple primary studies and reviews support ADRM1 as a 26S proteasome ubiquitin receptor required for efficient degradation of ubiquitin conjugates. Supporting Evidence: PMID:17139257 Knockdown of hRpn13 in 293T cells increases the cellular levels of ubiquitin conjugates and decreases the degradation of short-lived proteins PMID:18497817 Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain PMID:20471946 When wild-type hRpn13 was added to these proteasomes, the degradation of ubiquitinated cyclin B was strongly stimulated PMID:33729481 Three of these integral subunits (Rpn1, Rpn10 and Rpn13) bind to the conjugated ubiquitin tag on the substrate |
| GO:0061136 regulation of proteasomal protein catabolic process | NAS PMID:12032076 Properties of the hybrid form of the 26S proteasome containi... | ACCEPT | Summary: ADRM1 regulates proteasomal protein catabolism through substrate-recognition and UCHL5-recruitment mechanisms. Reason: Accept. The term is broad, but it fits ADRM1/Rpn13 as a ubiquitin receptor and UCHL5 recruiter whose loss or modification changes degradation of ubiquitin conjugates. Supporting Evidence: PMID:17139257 Knockdown of hRpn13 in 293T cells increases the cellular levels of ubiquitin conjugates and decreases the degradation of short-lived proteins PMID:20471946 When wild-type hRpn13 was added to these proteasomes, the degradation of ubiquitinated cyclin B was strongly stimulated PMID:24811749 Rpn13 ubiquitination strongly decreases the proteasome's ability to bind and degrade ubiquitin-conjugated proteins |
| GO:0071357 cellular response to type I interferon | NAS PMID:31380390 Regulation of Proteasome Activity by (Post-)transcriptional ... | MARK AS OVER ANNOTATED | Summary: The cited review discusses proteasome regulation in broad cellular contexts and does not establish ADRM1 as a direct type-I-interferon response effector. Reason: Mark as over-annotated. Type-I-interferon biology can regulate proteasome composition/activity, but ADRM1 should not receive a gene-level cellular response to type I interferon annotation from this broad proteasome-regulation review. Supporting Evidence: PMID:31380390 altered transcription of proteasomal subunits and activators |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | IDA GO_REF:0000052 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005634 nucleus | EXP PMID:16990800 A novel proteasome interacting protein recruits the deubiqui... | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005737 cytoplasm | EXP PMID:16990800 A novel proteasome interacting protein recruits the deubiqui... | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-174058 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-187574 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-188191 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5635854 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-68825 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-69600 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-8939801 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-8952408 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9762096 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005654 nucleoplasm | TAS Reactome:R-NUL-9604648 | ACCEPT | Summary: ADRM1-containing proteasomes are reported in the nucleus/nucleoplasm as part of the 26S proteasome distribution. Reason: Accept as a broad supported cellular location. Reactome nucleoplasm rows represent proteasome-catalyzed degradation events in nuclear pathways rather than independent ADRM1 pathway functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1168640 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234159 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1236970 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1504193 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-174105 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-174202 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-174203 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-174255 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-180573 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-180603 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-209061 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2130282 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-264458 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-353125 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-3640874 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-450466 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-4608855 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-4641256 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-4641260 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5362448 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5387392 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5607724 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5607731 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5610754 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5610758 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5610760 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5635868 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5658430 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5665854 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5665871 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5668481 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5668520 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5687112 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5689539 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-68948 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-69016 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-75825 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8850992 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8852354 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8854044 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8854071 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8866553 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8866858 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8932355 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956140 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956184 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8957265 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9755303 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9755306 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9766223 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-983150 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9907980 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9908026 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9908178 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9929352 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9929486 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9931314 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9934893 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9954728 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-NUL-212917 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-NUL-5610751 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0005829 cytosol | TAS Reactome:R-NUL-9011324 | ACCEPT | Summary: ADRM1-containing 26S proteasomes act in cytosolic protein degradation, and independent localization annotations also support cytosol/cytoplasm. Reason: Accept as a broad supported cellular location. The many Reactome cytosol rows reflect placement of the proteasome catalyst in substrate-degradation events; they should not be read as separate substrate-specific ADRM1 functions. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:33729481 The 26S proteasome is the major proteasome species in eukaryotes, responsible for proteolysis in the cytoplasm, in the nucleus |
| GO:0140678 molecular function inhibitor activity | EXP PMID:20471946 Structure of proteasome ubiquitin receptor hRpn13 and its ac... | MARK AS OVER ANNOTATED | Summary: The evidence describes intramolecular/autoregulatory inhibition of free hRpn13 ubiquitin-receptor activity by its C-terminal domain, not ADRM1 acting primarily as a standalone inhibitor. Reason: Mark as over-annotated. The autoinhibitory structural state is real, but the core function of ADRM1 is proteasomal ubiquitin receptor/deubiquitinase regulator; proteasome docking activates ubiquitin binding, so a broad inhibitor-activity annotation overstates the gene product role. Supporting Evidence: PMID:20471946 hRpn13 binding to the proteasome scaffolding protein hRpn2/S1 abrogates its interdomain interactions, thus activating hRpn13 for ubiquitin binding PMID:20471946 hRpn13's Uch37-binding domain can inhibit its activity as a ubiquitin receptor |
| GO:0000502 proteasome complex | IDA PMID:17323924 Mass spectrometric characterization of the affinity-purified... | ACCEPT | Summary: ADRM1 is a component of the 26S proteasome/regulatory-particle complex and is repeatedly recovered in proteasome structural and biochemical studies. Reason: Accept. Although the more precise PN-supported complex placement is the regulatory-particle base subcomplex, proteasome complex is a true broader cellular-component annotation for ADRM1. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:17139257 we discovered a novel 46-kDa (407 residues) subunit of its 19S regulatory complex PMID:29636472 Recognition of a ubiquitylated substrate is mediated principally by ubiquitin receptors Rpn10 and Rpn13, the base subunits within the holoenzyme PMID:33729481 Three of these integral subunits (Rpn1, Rpn10 and Rpn13) bind to the conjugated ubiquitin tag on the substrate |
| GO:0061133 endopeptidase activator activity | IDA PMID:16990800 A novel proteasome interacting protein recruits the deubiqui... | MODIFY | Summary: ADRM1 activates the proteasome-associated deubiquitinase UCHL5/UCH37, so the current endopeptidase activator term is too generic and mechanistically imprecise. Reason: Modify to deubiquitinase activator activity. The cited evidence is about recruiting/activating UCH37/UCHL5 deubiquitinating activity at 26S proteasomes, not a broad endopeptidase activator role. Proposed replacements: deubiquitinase activator activity Supporting Evidence: PMID:16990800 hRpn13 recruits UCH37, a deubiquitinating enzyme known to associate with 26 proteasomes PMID:16990800 loss of UCH37 proteins and decrease in deubiquitinating activity of 26S proteasomes PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity |
| GO:0002020 protease binding | IPI PMID:18922472 Distinct modes of regulation of the Uch37 deubiquitinating e... | MODIFY | Summary: The original protease-binding row is specifically UCHL5/UCH37 binding and should use the more informative ubiquitin-specific protease binding term. Reason: Modify to ubiquitin-specific protease binding. UCHL5/UCH37 is a proteasome-associated deubiquitinating enzyme, and ADRM1 binds/recruits it through the C-terminal DEUBAD region. Proposed replacements: ubiquitin-specific protease binding Supporting Evidence: PMID:16990800 hRpn13 recruits UCH37, a deubiquitinating enzyme known to associate with 26 proteasomes PMID:17139257 The C-terminal half of hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity PMID:18922472 The conserved DUB Uch37 is found on proteasomes PMID:20471946 Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37 |
| GO:0070628 proteasome binding | IDA PMID:18162577 Relative structural and functional roles of multiple deubiqu... | ACCEPT | Summary: ADRM1 binds/incorporates into the 26S proteasome regulatory particle through its Rpn13/Rpn2-associated receptor role. Reason: Accept as a direct molecular function. ADRM1 is a proteasome-associated ubiquitin receptor and interacts with the proteasome scaffolding protein Rpn2/PSMD1; this is also consistent with the PN regulatory-particle projection. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:17139257 we discovered a novel 46-kDa (407 residues) subunit of its 19S regulatory complex PMID:20471946 hRpn13 binding to the proteasome scaffolding protein hRpn2/S1 abrogates its interdomain interactions, thus activating hRpn13 for ubiquitin binding |
| GO:0000502 proteasome complex | IDA PMID:16990800 A novel proteasome interacting protein recruits the deubiqui... | ACCEPT | Summary: ADRM1 is a component of the 26S proteasome/regulatory-particle complex and is repeatedly recovered in proteasome structural and biochemical studies. Reason: Accept. Although the more precise PN-supported complex placement is the regulatory-particle base subcomplex, proteasome complex is a true broader cellular-component annotation for ADRM1. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:17139257 we discovered a novel 46-kDa (407 residues) subunit of its 19S regulatory complex PMID:29636472 Recognition of a ubiquitylated substrate is mediated principally by ubiquitin receptors Rpn10 and Rpn13, the base subunits within the holoenzyme PMID:33729481 Three of these integral subunits (Rpn1, Rpn10 and Rpn13) bind to the conjugated ubiquitin tag on the substrate |
| GO:0006368 transcription elongation by RNA polymerase II | IMP PMID:11818576 Human Elongator facilitates RNA polymerase II transcription ... | UNDECIDED | Summary: The cached abstract for the cited Elongator paper does not connect ADRM1 to RNA polymerase II transcription elongation, but this is an IMP (experimental) annotation whose full text is not available. Reason: This is an experimental (IMP) annotation citing PMID:11818576, which is abstract-only in our cache (full_text_available:false), so the supporting evidence cannot be verified. Per project guidelines, unverifiable experimental annotations should be UNDECIDED rather than REMOVE. Biologically the annotation is implausible - ADRM1/Rpn13 is a 26S proteasome ubiquitin receptor and is not a subunit of the human Elongator complex (ELP1-6); its supported role is proteasomal ubiquitin recognition and UCHL5/UCH37 regulation. Flagged for a curator with full-text access to confirm mis-attribution before removal. Supporting Evidence: PMID:11818576 human Elongator facilitates transcription by RNA polymerase II |
| GO:0043248 proteasome assembly | IDA PMID:16990800 A novel proteasome interacting protein recruits the deubiqui... | MARK AS OVER ANNOTATED | Summary: ADRM1 is recruited to 26S proteasomes and recruits UCHL5, but the cited evidence does not clearly show a proteasome assembly process role. Reason: Mark as over-annotated. The evidence supports proteasome binding/base-subcomplex membership and UCHL5 recruitment. It does not show ADRM1 catalyzing or directing proteasome assembly as a core biological process. Supporting Evidence: PMID:16990800 we describe the identification of Adrm1 as a novel proteasome interacting protein in mammalian cells PMID:16990800 hRpn13 recruits UCH37, a deubiquitinating enzyme known to associate with 26 proteasomes PMID:17139257 we discovered a novel 46-kDa (407 residues) subunit of its 19S regulatory complex |
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Download this section (compressed HTML)Q: Should the current ADRM1/Rpn13 GO annotation to proteasome regulatory particle, lid subcomplex be replaced by proteasome regulatory particle, base subcomplex in GOA/PAINT?
Suggested experts: Youdong Mao, Michael H. Glickman, Keiji Tanaka
Q: Should ADRM1 receive direct ubiquitin binding and deubiquitinase activator activity annotations in GOA to replace generic protein binding and endopeptidase activator activity?
Suggested experts: Kylie J. Walters, Alfred L. Goldberg, Keiji Tanaka
Experiment: Map endogenous ADRM1 contacts and cryo-EM density in substrate-engaged human 26S proteasomes, comparing wild-type ADRM1 with Pru-domain and DEUBAD-domain mutants for Rpn2, ubiquitin-chain, and UCHL5 association.
Hypothesis: Endogenous ADRM1 is a base/regulatory-particle ubiquitin receptor rather than a lid-subcomplex subunit.
Type: endogenous proteasome structural proteomics
Experiment: Rescue ADRM1-deficient cells with wild-type, ubiquitin-binding-defective, Rpn2-binding-defective, and UCHL5-binding-defective ADRM1 alleles, then quantify degradation of defined K48-ubiquitinated substrates and proteasome-associated UCHL5 activity.
Hypothesis: ADRM1 supports ubiquitin-dependent substrate degradation through both Pru-domain ubiquitin recognition and DEUBAD-dependent UCHL5 activation.
Type: catalytic-rescue degradation assay
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