VAMPIROME

Bottom line: a vampire bat has to keep its prey's blood liquid and its own
bite unnoticed, and it does so with a salivary secretome of anticoagulants,
protease inhibitors, vasodilators and immune modulators. Working from the
Vampirome transcriptome and proteome study, we mapped the reported transcripts
to UniProt, found that Desmodus rotundus has no Swiss-Prot entries at all in
this set, picked a 13-protein shortlist on hemostasis and immune relevance, and
reviewed them plus draculin (K9IMD0). Across 14 reviewed proteins there are 136
annotation rows: 64 ACCEPT, 29 MODIFY, 21 UNDECIDED, 15
MARK_AS_OVER_ANNOTATED, 4 NEW and 3 REMOVE. The large UNDECIDED share is the
honest result for an all-electronic, TrEMBL-only species where the underlying
experiments were done on other mammals. The fifteenth listed gene, CALCA
(vCGRP), is not yet reviewed: a transcriptome search found no matching peptide.

We did this because saliva proteins of blood-feeding animals are annotated
almost entirely by homology to their host counterparts, which imports the
host's biology wholesale. The C1-inhibitor homolog K9IYM3 is the clearest case:
its serpin ancestry brought peptidase activity and proteolysis, both removed,
and blood coagulation, hemostasis and fibrinolysis, all marked over-annotated.

Overview

Project to curate vampire bat (Desmodus rotundus; UniProt code DESRO) salivary gland proteins that modulate host hemostasis and immunity, based on the Vampirome transcriptome/proteome study. This project cross-links with PARASITE_IMMUNE_MODULATORS for broader parasite immune modulators work.

Slides

• Slides (Marp source: VAMPIROME-slides.md) — AI generated

Sources and project files

• Table 4 extract: projects/VAMPIROME/table4_extract.md
• UniProt mapping table: projects/VAMPIROME/uniprot_mapping.md
• UniProt mapping JSON: projects/VAMPIROME/uniprot_mapping.json
• Candidate list JSON (reviewed vs unreviewed): projects/VAMPIROME/uniprot_candidates.json
• Related project: projects/PARASITE_IMMUNE_MODULATORS.md

Seed Genes (DESRO)

• [x] Draculin (Lactotransferrin; UniProt: K9IMD0)
• [ ] CALCA

Vampirome candidates (prioritize Swiss-Prot)

Reviewed (Swiss-Prot)

None detected for DESRO in this candidate set (all mapped entries are UniProtKB unreviewed/TrEMBL).

Priority shortlist (start here; most immune/hemostasis relevant)

• [ ] t-plasminogen activator (UniProt: K9IJK6; entry K9IJK6_DESRO; transcript BatTrinityAbyss-499018)
• [ ] Kunitz-type protease inhibitor 2 (UniProt: K9IZA2; entry K9IZA2_DESRO; transcript BatTrinityAbyss-541822)
• [ ] Putative plasma protease c1 inhibitor (UniProt: K9IYM3; entry K9IYM3_DESRO; transcript DrSigp-SigP-532391)
• [ ] Deoxyribonuclease-1-like 1 (UniProt: K9J287; entry K9J287_DESRO; transcript BatTrinityAbyss-532109)
• [ ] C-C motif chemokine (UniProt: K9IFY6; entry K9IFY6_DESRO; transcript BatTrinityAbyss-506850)
• [ ] Lymphotoxin-alpha (UniProt: K9IWR0; entry K9IWR0_DESRO; transcript BatTrinityAbyss-86412)
• [ ] Beta-defensin 1 (UniProt: K9IFT7; entry K9IFT7_DESRO; transcript BatTrinityAbyss-401005)
• [ ] lysozyme (UniProt: K9IWH5; entry K9IWH5_DESRO; transcript BatTrinityAbyss-500942; gene LYZ)
• [ ] Tumor necrosis factor-inducible gene 6 protein (UniProt: K9IIP0; entry K9IIP0_DESRO; transcript BatTrinityAbyss-527888)
• [ ] A disintegrin and metalloproteinase with thrombospondin motifs 1 (UniProt: K9IUF6; entry K9IUF6_DESRO; transcript BatTrinityAbyss-517665)
• [ ] Natriuretic peptides B (UniProt: K9IWC0; entry K9IWC0_DESRO; transcript BatTrinityAbyss-538594)
• [ ] Putative scp crisp: scp-like extracellular (UniProt: K9IWX5; entry K9IWX5_DESRO; transcript BatTrinityAbyss-495870)
• [ ] Dipeptidyl peptidase 4 (UniProt: K9J2R0; entry K9J2R0_DESRO; transcript BatTrinityAbyss-561424)

Unreviewed (TrEMBL)

• [ ] 2-phosphoxylose phosphatase 1 (UniProt: K9IKM9; entry K9IKM9_DESRO; transcript BatTrinityAbyss-508800)
• [ ] 5'-nucleotidase domain-containing protein 1 (UniProt: K9IKC7; entry K9IKC7_DESRO; transcript BatTrinityAbyss-537653)
• [ ] A disintegrin and metalloproteinase with thrombospondin motifs 1 (UniProt: K9IUF6; entry K9IUF6_DESRO; transcript BatTrinityAbyss-517665)
• [ ] Acid sphingomyelinase-like phosphodiesterase (UniProt: K9IL01; entry K9IL01_DESRO; transcript BatTrinityAbyss-549118)
• [ ] Beta-defensin 1 (UniProt: K9IFT7; entry K9IFT7_DESRO; transcript BatTrinityAbyss-401005)
• [ ] C-C motif chemokine (UniProt: K9IFY6; entry K9IFY6_DESRO; transcript BatTrinityAbyss-506850)
• [ ] Deoxyribonuclease-1-like 1 (UniProt: K9J287; entry K9J287_DESRO; transcript BatTrinityAbyss-532109)
• [ ] Dipeptidyl peptidase 4 (UniProt: K9J2R0; entry K9J2R0_DESRO; transcript BatTrinityAbyss-561424)
• [ ] Epoxide hydrolase (UniProt: K9IKD6; entry K9IKD6_DESRO; transcript BatTrinityAbyss-508632)
• [ ] Kunitz-type protease inhibitor 2 (UniProt: K9IZA2; entry K9IZA2_DESRO; transcript BatTrinityAbyss-541822)
• [ ] Lymphotoxin-alpha (UniProt: K9IWR0; entry K9IWR0_DESRO; transcript BatTrinityAbyss-86412)
• [ ] Metalloproteinase inhibitor 1 (UniProt: K9IGJ9; entry K9IGJ9_DESRO; transcript BatTrinityAbyss-37180)
• [ ] Metalloproteinase inhibitor 3 (UniProt: K9IHC5; entry K9IHC5_DESRO; transcript BatTrinityAbyss-534229)
• [ ] Natriuretic peptides B (UniProt: K9IWC0; entry K9IWC0_DESRO; transcript BatTrinityAbyss-538594)
• [ ] Putative alpha-1-antichymotrypsin (UniProt: K9IXP7; entry K9IXP7_DESRO; transcript BatTrinityAbyss-518161)
• [ ] Putative cystatin-m (UniProt: K9IWH8; entry K9IWH8_DESRO; transcript BatTrinityAbyss-41885)
• [ ] Putative disintegrin and metalloproteinase (UniProt: K9IZP9; entry K9IZP9_DESRO; transcript BatTrinityAbyss-521171)
• [ ] Putative iggfc-binding protein (UniProt: K9J450; entry K9J450_DESRO; transcript DrSigp-SigP-495835)
• [ ] Putative neuroserpin is a inhibitory member of (UniProt: K9J0L7; entry K9J0L7_DESRO; transcript BatTrinityAbyss-548577)
• [ ] Putative pituitary adenylate cyclase-activating (UniProt: K9IGD6; entry K9IGD6_DESRO; transcript BatTrinityAbyss-500584)
• [ ] Putative plasma protease c1 inhibitor (UniProt: K9IYM3; entry K9IYM3_DESRO; transcript DrSigp-SigP-532391)
• [ ] Putative salivary lipocalin (UniProt: K9IRT4; entry K9IRT4_DESRO; transcript BatTrinityAbyss-466603)
• [ ] Putative salivary lipocalin (UniProt: K9IHB8; entry K9IHB8_DESRO; transcript BatTrinityAbyss-495622)
• [ ] Putative salivary lipocalin (UniProt: K9IWR5; entry K9IWR5_DESRO; transcript BatTrinityAbyss-495624)
• [ ] Putative salivary lipocalin (UniProt: K9IQU6; entry K9IQU6_DESRO; transcript BatTrinityAbyss-495626)
• [ ] Putative salivary lipocalin (UniProt: K9IWP0; entry K9IWP0_DESRO; transcript BatTrinityAbyss-495631)
• [ ] Putative salivary lipocalin (UniProt: K9IYU4; entry K9IYU4_DESRO; transcript BatTrinityAbyss-496761)
• [ ] Putative scp crisp: scp-like extracellular (UniProt: K9IWX5; entry K9IWX5_DESRO; transcript BatTrinityAbyss-495870)
• [ ] Putative secreted mucin (UniProt: K9IGW6; entry K9IGW6_DESRO; transcript DrSigp-SigP-36965)
• [ ] Putative secreted protein precursor (UniProt: K9IFW5; entry K9IFW5_DESRO; transcript BatTrinityAbyss-36340)
• [ ] Putative serpin (UniProt: K9IJG1; entry K9IJG1_DESRO; transcript BatTrinityAbyss-25903)
• [ ] Serpin B6 (UniProt: K9J5D5; entry K9J5D5_DESRO; transcript BatTrinityAbyss-543253)
• [ ] Tumor necrosis factor-inducible gene 6 protein (UniProt: K9IIP0; entry K9IIP0_DESRO; transcript BatTrinityAbyss-527888)
• [ ] lysozyme (UniProt: K9IWH5; entry K9IWH5_DESRO; transcript BatTrinityAbyss-500942; gene LYZ)
• [ ] t-plasminogen activator (UniProt: K9IJK6; entry K9IJK6_DESRO; transcript BatTrinityAbyss-499018)

Unmapped Vampirome transcripts (no TSA/UniProt hit yet)

• vCGRP (calcitonin gene-related peptide-like; vasodilatory peptide). Reported peptide sequence:
  SCNTATCVTHRLAGLLSRSGGVVSSDFTPTDTGSNSY (Toxins 2019, Kakumanu et al.). Mapping to
  DESRO transcript/UniProt pending.
• BatTrinityAbyss-321620
• BatTrinityAbyss-429618
• BatTrinityAbyss-489254
• BatTrinityAbyss-499100
• BatTrinityAbyss-499228
• BatTrinityAbyss-500441
• BatTrinityAbyss-509950
• BatTrinityAbyss-523646
• BatTrinityAbyss-8258
• DrSigp-SigP-210264

STATUS

2026-01-21

• [x] Create Vampirome project structure and cross-link to PARASITE_IMMUNE_MODULATORS
• [x] Import Table 4 extract and UniProt mapping artifacts
• [x] Build candidate list with Swiss-Prot prioritization (none found)
• [x] Select priority shortlist (13 candidates)
• [ ] Expand seed list with any additional vampire bat modulators supplied by user
• [x] Fetch UniProt/GOA data for prioritized candidates
• [x] Deep research (falcon) for 13 shortlisted candidates
• [ ] Review existing annotations (annotation-reviewer)
• [ ] Synthesize core functions

2026-01-22

• [x] Add core_functions and descriptions for Draculin and three priority candidates
• [x] Add deep-research support snippets for core functions where available
• [x] Create notes files for Draculin (K9IMD0), K9IZA2, K9IYM3, and K9J287
• [x] Create notes files for remaining shortlist candidates (K9IJK6, K9IFY6, K9IWR0, K9IFT7, K9IWH5, K9IIP0, K9IUF6, K9IWC0, K9IWX5, K9J2R0)

NOTES

2026-01-21

• Created VAMPIROME project and moved Vampirome artifacts into projects/VAMPIROME/.
• UniProt mapping indicates all current DESRO candidates are UniProtKB unreviewed (TrEMBL); no Swiss-Prot entries detected.
• Cross-link with projects/PARASITE_IMMUNE_MODULATORS.md added.
• Selected a 13-gene priority shortlist focused on hemostasis/immune modulation and fetched UniProt/GOA data (K9IJK6, K9IZA2, K9IYM3, K9J287, K9IFY6, K9IWR0, K9IFT7, K9IWH5, K9IIP0, K9IUF6, K9IWC0, K9IWX5, K9J2R0).
• Falcon deep-research completed for all 13 shortlisted candidates; needed extended timeouts for some runs.
• Completed annotation review for Draculin (K9IMD0 / LTF).
• Completed annotation reviews for K9IJK6, K9IZA2, K9IYM3, K9J287, and K9IWR0.
• Completed annotation reviews for K9IFT7, K9IWH5, K9IIP0, K9IUF6, and K9IWC0.
• Completed annotation reviews for K9IWX5 and K9J2R0.

2026-01-22

• Added core_functions/description edits for Draculin (K9IMD0), K9IZA2, K9IYM3, and K9J287.
• Added deep-research support snippets for core functions where available.
• Created notes files for K9IMD0, K9IZA2, K9IYM3, and K9J287.
• Created notes files for remaining shortlisted candidates (K9IJK6, K9IFY6, K9IWR0, K9IFT7, K9IWH5, K9IIP0, K9IUF6, K9IWC0, K9IWX5, K9J2R0).
• Started CALCA/vCGRP mapping via transcriptome search; no peptide matches in RefSeq or TSA GABZ01 (see CALCA-bioinformatics RESULTS).