Forty horse genes for ProtNLM evaluation

EVALUATIONML_PREDICTIONS

Forty horse genes for ProtNLM evaluation

40 selected horse genes with 89 GO predictions and 17 function descriptions.
The paired reviews evaluate these horse predictions using exact sequence evidence
and characterized mammalian counterparts. The selection is based
on informative functional claims and biological diversity. Every gene has GO or
function-text output; protein-name predictions were not a selection criterion.

Download the 40-gene list ·
All 106 GO/function outputs ·
Current protein sequences ·
Selection manifest

27 genes have GO predictions, 17 have function descriptions, and four have both.
The list includes catalytic/regulatory distinctions, substrate and pathway
specificity, taxonomic context, localization and developmental claims. The focus
column records the review question, not a correctness verdict. Each target is paired
with a human review and evaluated against primary evidence and its exact current
horse sequence. See the review findings and evidence gaps.

# Horse review Human review Accession GO Function text Review focus
1 VAPA VAPA A0A3Q2H1L9 0 1 Test sperm-crawling narrative and taxonomic context
2 CAPSL CAPSL A0A3Q2I3U9 0 1 Test venom-secretion narrative
3 MYL10 MYL10 A0A9L0TJE1 0 1 Test venom-secretion narrative on a different protein family
4 KRIT1 KRIT1 A0A9L0SR44 0 1 Test piRNA and germline narrative against target identity
5 CTDSP2 CTDSP2 F7A4N8 1 0 Resolve predicted kinase activity versus target catalytic class
6 PEA15 PEA15 A0A9L0RWM8 1 0 Test carbohydrate-transport process assignment
7 ALDH7A1 ALDH7A1 A0A9L0RRL6 1 0 Resolve precise aldehyde-dehydrogenase substrate specificity
8 GHSR GHSR F6QF00 0 1 Test oxytocin-receptor narrative and ligand specificity
9 ALG5 ALG5 A0A5F5PM72 0 1 Resolve sugar donor and dolichol-glycosylation reaction
10 HSPD1 HSPD1 F6Z587 0 1 Distinguish chaperonin complexes and transferred client biology
11 CXCR3 CXCR3 A0A9L0T1D1 0 1 Resolve chemokine ligand class and specificity
12 DARS2 DARS2 A0A9L0SB67 0 1 Test tRNA-Asn charging and organellar substrate specificity
13 GPAM GPAM A0A9L0TTC1 0 1 Distinguish acyl-ACP from acyl-CoA donor chemistry
14 HSPA4 HSPA4 A0A9L0S5Z5 0 1 Resolve ribosome-associated chaperone-family and complex transfer
15 DUOX1 DUOX1 A0A9L0SQG9 4 1 Separate hydrogen peroxide production, catabolism and thyroid context
16 MTMR9 MTMR9 A0A9L0T3C1 7 0 Separate phosphatase regulation and binding from intrinsic catalysis
17 PTPRN2 PTPRN2 A0A9L0T4W6 1 0 Assess dephosphorylation process for a phosphatase-like protein
18 DNMT3L DNMT3L A0A9L0T837 2 0 Compare regulatory function with active DNA methyltransferase
19 IRAK3 IRAK3 A0A3Q2HDT6 0 1 Assess receptor-signaling narrative and inactive-kinase context
20 PPP4R4 PPP4R4 A0A9L0S961 3 0 Separate phosphatase-regulator function from developmental transfer
21 CDK7 CDK7 A0A9L0R074 2 0 Evaluate specific kinase activity and phosphorylation process
22 MAP2K2 MAP2K2 A0A9L0SHX8 1 0 Evaluate conserved kinase process and specificity
23 ZDHHC23 ZDHHC23 A0A9L0T4E4 1 0 Evaluate specific protein S-palmitoyltransferase activity
24 CH25H CH25H F6T000 3 0 Evaluate oxidoreductase and lipid-biosynthesis specificity
25 BCAT2 BCAT2 A0A9L0TSN4 0 1 Evaluate branched-chain amino-acid catabolic narrative
26 SIRT5 SIRT5 F6S899 1 1 Evaluate deacylation substrate classes and weak in-vitro activity caveat
27 USP8 USP8 A0A9L0T7K6 1 1 Separate deubiquitination function from protein-catabolism process
28 OMA1 OMA1 A0A9L0R9P8 1 0 Evaluate proteolysis prediction and more specific mitochondrial role
29 EFR3A EFR3A A0A9L0S4L8 1 0 Evaluate protein recruitment to plasma membrane
30 CACNB3 CACNB3 A0A5F5PZM5 1 0 Distinguish ion-channel regulation from ion transport catalysis
31 SHLD2 SHLD2 A0A9L0RGD6 6 1 Evaluate DNA-repair directionality, class switching and localization
32 GEMIN5 GEMIN5 A0A9L0R5P7 6 0 Compare RNA-binding and translation claims with ubiquitination
33 AFAP1L2 AFAP1L2 A0A9L0RQI4 9 0 Evaluate adaptor binding, kinase activation and cytokine processes
34 OLFML2A OLFML2A A0A9L0SKW1 4 0 Separate extracellular-matrix location, binding and organization
35 CC2D2A CC2D2A A0A5F5PJ44 4 0 Evaluate developmental-process transfer and ciliary mechanism
36 WDPCP WDPCP A0A3Q2KRK8 4 0 Evaluate cilium and cytoskeleton localization with projection organization
37 TRAF2 TRAF2 F7BIV4 5 0 Evaluate receptor binding, complex membership and immune context
38 WEE1 WEE1 F6TY09 7 0 Separate kinase-related processes from oocyte-specific context
39 DYNLT2B DYNLT2B A0A9L0SWY1 10 0 Evaluate ciliary transport, dynein binding and localization claims
40 DNMT3A DNMT3A A0A9L0TK01 2 0 Compare gene-expression regulation with DNMT3L on the same output terms

Identity and evidence

There is one accession per selected gene label and no duplicate current protein
sequences. SHLD2 uses A0A9L0RGD6; the additional release record A0A3Q2HUD4 is not a
second benchmark gene. Symbols come from the frozen official accession list.
Some current UniProt records omit their gene-name field; that absence is retained
in the CSV rather than filled from the model's predicted name.

The ordinary UniProt record snapshot
provides species, sequence versions, lengths and sequence checksums. All 40 are
horse records and all current lengths match the release list. This does not prove
that every record is a complete protein or that the prediction-time sequence is
unchanged; examine gene models and domain completeness during review.

The CSV identifies existing human, mouse and rat review files with matching gene
symbols as evidence-search leads. These are not verified orthology assignments or
independent biological validation. Establish the counterpart and trace its
experimental/analytical evidence before transferring a claim to horse.

This is a targeted, retrospective cohort, not a random horse sample or an estimate
of whole-proteome accuracy. A later mammalian benchmark can grow from these
horse-anchored cases after orthology and evidence are established. The original
ARGO-ProtNLM-50 is unchanged.

Benchmark design and census ·
ProtNLM evaluation project